Journal: iScience
Article Title: Reactive oxygen species-responsive hydrogel loaded with melatonin as a minimally invasive treatment for myocardial I/R injury
doi: 10.1016/j.isci.2025.112834
Figure Lengend Snippet: PT-MLT hydrogel improves inflammatory response in rats with MIRI (A) A schematic showing the SD rats study design. (B) Photograph showing the animal models of reserve line, LAD ligation, and intramyocardial injection. (C) Images of HE staining of myocardium after 5 days. (D) The levels inflammatory factors of IL-6 of SD rat serum after 3 days. (E) The levels inflammatory factors of IL-1β of SD rat serum after 3 days. (F) The levels inflammatory factors of TNF-α of SD rat serum after 3 days. (G) Immunofluorescence fluorescence staining images of IL-1β in rat myocardial tissue after 5 days. (H) Fluorescence intensity of immunofluorescence staining. ( n = 3 independent biological replicates; ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05).
Article Snippet: Hematoxylin eosin (HE) staining solution kit , Solarbio , Cat#C0105S.
Techniques: Ligation, Injection, Staining, Immunofluorescence, Fluorescence